When attempting to connect to a published application the user may be prompted to enter/ provide their logon credentials again.
There are two possible causes for this issue.
Resolution 1:
If server hosting the application(s) is a Terminal Server, check the settings below. If the application is published from a VDI, skip this resolution step.
1. Select Start | Administrative Tools | Terminal Services Configuration
2. Right Click on RDP-Tcp and select Properties
3. Select the Logon Settings Tab
4. Ensure that the option "Always Prompt For Password" is not selected
,
Resolution 2.
1. Navigate to HKLM\Software\Microsoft\Windows NT\ CurrentVersion\Winlogon\GinaDLL
2. The GinaDLL string value should have a data value of pngina.dll
3. If it does not exist, create the key with the appropriate data(it is a string value).
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