The checkbox won’t be disabled if you delete that password from the file location.
But if needed to key in the password each time connecting, this can be done by the following:
1. Disconnect and open the Spotlight connection properties details -> remove the passwords and click OK to save the profile;
2. Find the connection profile by default location, and set the profile as Read-only;
3. Restart Spotlight connection, then you should need to enter password each time.
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