Is it possible to assign users application sets depending on the method used to login, for example, if a user logs in via Web Access using Two Factor Authentication they may be given access to a different set of applications than a user using only a username and password.
This is possible in vWorkspace 7.5 and later, by employing the "Advanced Targets" feature.
Please see page 102 of the vWorkspace 7.5 Administration guide for more information on Advanced Targets.
It is not currently possible to force specific users to use Two Factor Authentication. It is possible to force Two Factor Authentication at the farm level but this applies to all users.
Administrators looking to force specific users to use Two Factor Authentication can however remove all applications assigned to these users so that when the user logs in, no applications or desktops are available. Applications and desktops can then be assigned via Advanced Targets if Two Factor Authentication is used.